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Question:I had a paper with 30 questions of DNA replication, but I couldn't get a few answers. I really appreciate your help.
1) how does the nucleotide in one chain of DNA compare with the other chain of DNA?
2) What enzymes help separate the 2 strands of nucleotides on DNA? What bonds do they break?
3) What makes up the "steps" of a DNA molecule?
4) What type of bonds holds the DNA bases together? Are they strong or weak bonds.
Answers:1. The are complementary bases bonded to each other. G with C, A with T.
2. Helicase(s). Hydrogen bonds.
3. Nucleotides (the A, G, T, Cs)
4. Hydrogen bonds...weak. Which is why helicase can break them.
Question:1.Which of the following would represent the "transformation principal" first proposed by Griffith through his studies on Streptococcus pneumoniae bacterium?
2.Which of the following is the highest (or most complex) level of structure for genetic material?
3.Which of the following is FALSE when comparing RNA and DNA?
Both are produced by phosphodiester linkages.
Both are composed of phosphate groups.
Both are composed of sugars.
Both are composed of identical nucleotides.
4.A DNA specimen that contains 30% guanine has ________ thymine.
The amount of thymine cannot be determined.
5.In producing a strand of DNA the nucleotides combine to form what type of bond?
6.During DNA replication, all of the following proteins are important for separating the DNA strands and allowing movement of the replication fork EXCEPT
single-stranded binding proteins.
both helicase and topoisomerase.
7.Which of the following statements about DNA replication is INCORRECT?
It begins at multiple origins of replication sites along eukaryotic chromosomes.
It proceeds with the addition of new nucleotides to the 3 end of a growing DNA strand.
It is powered by the hydrolysis of ATP.
Each strand within the DNA double helix is used as a template for synthesis of a new strand.
It requires that each strand in the double helix be separated from the other.
8.Where would one expect to find the most telomerase?
male germ cells that give rise to gametes
skin cells from a 60-year-old individual
skin cells from a 2-year-old individual
a nerve cell from a 60-year-old individual
a nerve cell from a 2-year-old individual
9.Which of the following statements best describes DNA polymerase?
It is an enzyme required to produce a primer needed for DNA replication.
It is an enzyme that catalyzes the addition of nucleotides to the 5 end of a growing DNA strand.
It is an enzyme that requires the presence of a small double-stranded nucleic acid primer to catalyze the addition of nucleotides to the 3 end of a growing DNA strand.
It is an enzyme required to glue pieces of DNA fragments together.
It is an enzyme that polymerizes to the DNA and keeps the replication fork open.
10.Which molecule is responsible for separating double-stranded DNA into single strands?
single-stranded binding proteins
Both are composed of a phosphate group, a pentose sugar, and a nitrogenous base.
3. Both are composed of identical nucleotides.
6. DNA polymerase
7. It is powered by the hydrolysis of ATP
8. Telomerase is important in dividing cells, to make sure all the genetic information is retained, so I think this is the germ cell that gives rise to gametes. Could be the 2 year old skin cell, since skin cells have a high turnover rate.
9.It is an enzyme that requires the presence of a small double-stranded nucleic acid primer to catalyze the addition of nucleotides to the 3 end of a growing DNA strand.
Question:Put the following steps of DNA replication in chronological order.
1. Single-stranded binding proteins attach to DNA strands.
2. Hydrogen bonds between base pairs of antiparallel strands are broken.
3. Primase binds to the site of origin.
4. DNA polymerase binds to the template strand.
5. An RNA primer is created.
Answers:I am pretty sure its B......
Question:Can anyone help me with this question?
What would be the effect on DNA replication of mutations that destroyed each of the following activities in the specified bacterial enzymes?
(A) DNA polymerase III , 3' -> 5' exonuclease activity
(B) DNA polymerase I, 5' -> 3' exonuclease activity
(C) DNA ligase
Answers:Start with D, helicase. With out the opening of the strands, so that transcription is physically possible, you would not have to consider any of the following steps. Now work backwards.